||[Nov. 30th, 2005|05:33 pm]
Looking at small things
I'll be starting my third year EM project next week and thought some of you might be interested. What I am doing is an SEM investigation of human ciliated epithelial cells. What I'm looking at specifically is the best way to image these things to avoid artefacts and image distortions.|
I have some pots of human lung recovered from lung resection operations carried out in the local hospital and will be testing the effects of critical point drying, freeze drying and fully hydrated cells on a peltier cold stage in low-vacuum SEM. The aim is to find the ideal way for preparing these cells for use in further studies. The aim is to provide a good guide for studies involving abnormalties, etc, that could potentially be obscured by artefacts.
I wonder if anyone here has experience working with ciliated epithelia and might have some tips for imaging. Particularly, does anyone have any insights about what method is likely to produce the most accurate images?